Text/Yangcheng Evening News All-Media Reporter Lin Qingqing
Correspondent Hu Xuejiao Zhang Lanxi
Graph/Zhao Yunhu
The identification can be made in about 1 hour! The Guangdong team published the nucleic acid detection technology for rapid typing of new crown variants, which can help the rapid shunting of suspected new crown patients and the accurate typing of Delta variants.
The reporter learned from the Guangdong Provincial People’s Hospital on the 24th that recently, the team of Professor Gu Bing of the Laboratory Department of the Guangdong Provincial People’s Hospital and the team of Professor Zhang Lei of South China University of Technology published in the authoritative magazine “Emerging Microbes & Infections” in the field of infection in the 1st District of the Chinese Academy of Sciences Authored a paper entitled “RT-LAMPassayforrapiddetectionoftheR203MmutationinSARS-CoV-2Deltavariant”.
In this study, a detection method based on RT-LAMP was developed to identify the level of single base mutation. Two pairs of specific primers were designed for the conserved region of SARS-CoV-2N gene and the Delta-specific mutation R203M region. (conservedprimerset and R203primerset), when the CqR203M/Cqconserved threshold is 1.80, the rapid detection and differentiation of the new coronavirus Delta mutant strain and wild strain can be achieved, and clinical verification has been carried out, and the typing accuracy rate has reached 100%.
Establishment of RT-LAMP method for detection of typical mutation sites of new crown Delta
This method realizes The identification of Delta variant strains and the real-time monitoring of SARS-CoV-2 infection in about 1 hour can be carried out on a conventional fluorescence quantitative PCR detection platform. means.
Cq-ratio based RT-LAMP technology for detection and typing of COVID-19 clinical samples